Speakers > Pascal Fries
Pascal Fries ESI Frankfurt, Germany Multi-electrode recordings and optogenetic activation in awake marmosets
The common marmoset has emerged as a key model in neuroscience. Marmosets are small in size, show great potential for genetic modification and exhibit complex behaviors. Thus, it is necessary to develop technology that enables monitoring and manipulation of the underlying neural circuits. In this talk, I will present our approach to record and optogenetically manipulate neural activity in the awake, behaving marmoset. The design utilizes a light-weight, 3D printed titanium chamber that can house several high-density silicon probes for semi-chronic recordings, while enabling simultaneous optogenetic stimulation. We demonstrate the application of our method by recording multi- and single-unit data from areas V1 and V6 with 192 channels simultaneously, and show for the first time that optogenetic activation of excitatory neurons in area V6 can influence behavior in a detection task. Furthermore, I will present additional technical developments that address the specific behavioral needs of the marmoset, namely, head-free eye tracking and efficient receptive field mapping.
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